Wednesday, February 27, 2013

Leishmaniasis


Leishmaniasis
These haemoflagellates of the genus Leishmania are parasites of invertebrates and vertebrates that use small biting sand flies (Phlebotomus), such as P. sargenti, P. papatasii, P. argentipes and many others, depending on the geographical area as vectors. The leishmanias are rounded or oval parasites with a morphologically complex life cycle consisting of a promastigote with a free flagellum and amastigote that has no flagellum.
Human leishmaniasis is scattered over wide areas of the globe, from China across Asia to IndiaIranAfghanistan, the Middle and Near East, the Mediterranean basin, into Sudan and Ethiopia and East and West Africa. In the New World, it extends fromMexico to the northern part of Argentina. There is no continuity in the distribution over these wide areas nor is a single disease involved. The distribution is determined by the kinds of mammals that serve as its hosts and by the distribution of the sand fly.
The diseases attributed to Leishmania range from a mildly inconvenient lesion of the skin, known as Oriental sore, to a serious disease involving the liver and spleen, known as kala-azar. In South America, one form of leishmaniasis (mucocutaneous) extensively involves the mucous membranes of the mouth, nose and pharynx, as well as the skin but does not affect the viscera.
Leishmaniasis is a zoonotic disease that is maintained in nature by many animals including dogs, rodents, wolves, foxes, jackals, raccoons, sloths and marsupials. Numerous species of Leishmania have been described in mammals and of these three are generally accepted as human parasites; two of these cause cutaneous leishmaniasis and the third causes visceral leishmaniasis or kala-azar.
Life cycle, symptomatology and epidemiology
The amastigote forms of Leishmania are ingested by sand flies when they feed on the blood and tissue fluid of vertebrates. Compared with trypanosomes, development of Leishmania in the sand fly seems quite simple, involving the transformation of the amastigote forms to promastigotes. These promastigotes multiply in the digestive canal of the insect and transform into infective promastigotes which are injected into a new vertebrate host when the fly feeds again. 
Life cycle

In the vertebrate the promastigotes enter host macrophage cells, roundup and lose their flagella, forming the so-called Leishman bodies or amastigote forms that are the characteristic forms found in the vertebrate. The parasites multiply and increase within the host cells until they cause them to rupture. The rapture of the host cells liberates more parasites that are engulfed by other phagocytic cells.
Table. Leishmania species and main characteristics associated with infections
Species
Characteristics
Major foci
Old World
L. aethiopica
Cutaneous, self-healing, also diffuse, zoonotic
Eastern Africa
L. donovani
Visceral, kala-azar, anthroponotic
IndiaEastern Africa
L. infantum
Visceral, zoonotic (dogs)
EuropeNorth Africa
L. major
Cutaneous, self-limiting, rural, zoonotic
Mediterranean basin and Western Asia
L. tropica
Cutaneous, self-healing, with possible recurring satellite lesions, urban, anthroponotic
Mediterranean basin and Western Asia andAfrica
New World
L. braziliensiscomplex
Cutaneous and mucocutaneous, zoonotic
Latin America
L. chagasi
Visceral, zoonotic (dogs)
Latin America
L. mexicanacomplex
Cutaneous, and a few mucocutaneous reported inPanama, zoonotic
Latin America
Cutaneous leishmaniasis (Oriental Sore)
Cutaneous Leishmaniasis is characterised by sores on the face, arms and legs especially in children. The aetiological agent isLeishmania tropica, a parasite that has a wide distribution in AsiaMiddle East and Africa.
The classical lesion of cutaneous leishmaniasis is the Oriental Sore, which is about 5 to 15 cm in diameter and takes several months to develop following infection. Incubation period is usually between 2 to 7 months or longer. The infection is usually accompanied by spontaneous healing of the sore after 9 to 12 months, sometimes leaving unsightly scars on the skin. There is increasing evidence that the infection is followed by permanent immunity.
The sores do not usually contain pus except when they have been invaded by bacteria. Classical sores are found from the Mediterranean region to India and southwestern Asia and parts of Africa. Another form of Oriental sore is the relatively wet one that attacks the lymph nodes. This has a shorter incubation period than the dry Oriental sore. It is probably a zoonosis since it occurs in rural areas where its reservoirs are dogs, ground squirrels and gerbils.
Mucocutaneous leishmaniasis (Espundia)
Mucocutaneous leishmaniasis is a chronic disease that occurs in Central and South America. The aetiological agent isLeishmania braziliensis and the most severe form of the disease is called espundia which is endemic in the jungles of South America, particularly PeruBolivia and BrazilLeishmania braziliensis prefers to attack the mucus membranes of the mouth and the nasopharynx. The infection is usually complicated by bacterial and infections and may result in the complete destruction of the tissues of the mucus membranes including the associated cartilage, leading to grotesque deformation of the nasopharyngeal region and the palate.
Oriental sore (A and B) and espundia (C)
Visceral Leishmaniasis (kala-azar)
Visceral leishmaniasis is caused by L. donovani, a parasite similar to both Ltropica and Lbraziliensis. Visceral leishmaniasis exists in ChinaIndiaAfghanistanSudanEthiopia and East Africa, South and Central America. It attacks the reticuloendothelial cells of the spleen, liver, bone marrow and visceral lymphatic nodes. This is a rural disease with dogs, foxes, rodents and other mammals acting as its reservoirs.
Biting sandflies introduce promastigotes into the dermal region of the skin. The promastigotes enter the blood and lymph vessels and make their way to the liver, spleen, and bone marrow via lymph and blood. As the parasites divide and increase in numbers, they cause the rupture of the macrophages and release of more parasites that become available to infect more macrophages. The destruction of the macrophages continues until their numbers are considerably reduced.
To counteract the loss of macrophages, the bone marrow is activated to produce more macrophages at the expense of other important cells such as polymorphonuclear cells. The resulting monocytosis and leucopoenia weaken the defence system of the body very badly. The result is that many of the victims of kala-azar succumb to infections that they would normally resist.
Clinical kala-azar develops when the liver, spleen, kidney, and bone marrow are infected. Very often kala-azar is fatal if not treated. In other people, however, the symptoms may assume a chronic course.
The symptoms of kala-azar include headaches, irregular bouts of fever with a temperature (which can range from 36 oC to 40 oC) and oedema of the face, trunk and feet. Other symptoms include substantial weight loss, splenomegaly, hepatomegaly, and anaemia, bleeding from the gums, lips, nose and intestinal mucosa.
Leishmania donovani exhibits geographical host-relationships. In China, kala-azar has dogs as its reservoirs that suffer from extensive lesions. In India, kala-azar has no zoonotic features because it is transmitted easily from man to man by sand flies living in moist vegetation in shaded areas near homes.
In the Mediterranean region where dogs are important reservoirs, children are usually more affected than the adults. In theSudan, mucocutaneous leishmaniasis and kala-azar are present and both are caused by L. donovani. In the Sudan as in theAmericas, mucocutaneous leishmaniasis affects the upper respiratory tract and oral regions of the body. However, in the Sudan, it is maintained by several rodents such as Arvicanthis niloticus (the Nile grass rat), Acomys albigena (the spiny mouse), the genet and the serval. In the semi - arid northern districts of Kenya and the districts of Karamoja in Uganda, sandflies live in anthills and feed on rodents and dogs.
Diagnosis
In cutaneous leishmaniasis, the presence of parasites can be demonstrated in blood smears made from skin scrapings, aspiration of juice from the wound or biopsy. Alternatively, the scrapings from the lesion can be cultured or inoculated into hamsters and examined for the presence of amastigotes. In kala-azar, a biopsy from the bone marrow or spleen can be examined for the presence of amastigotes, cultured on a suitable media or inoculated into hamsters before being examined. In either case, the presence of amastigotes confirms the infection.
Treatment
Proper parasitological identification of the parasites is essential for correct treatment. This is because the treatment of leishmaniasis is long and the drugs are toxic. Cutaneous leishmaniasis is usually self-limiting and most of the wounds heal within 6-9 months. The infection can therefore be left to take its course until it heals, unless the wounds are disfiguring because of bacterial involvement. In many parts of southwest Asia, children are exposed to infection or given live virulent injections of the parasite in order to induce immunity that will protect them against further infection and thus avoid disfiguring lesions and scars on the face.
A case of kala-azar, with enlarged liver and spleen
LeishmaniasisLG
Mucocutaneous leishmaniasis and kala-azar are more serious than cutaneous leishmaniasis because they are not only incapacitating they can also be fatal. The first line of treatment involves the administration of pentavalent antimonials, such as stibogluconate, or meglumine antimoniate. The second line drugs if the antimonials fail are amphoterin B and pentamidine. If untreated, kala-azar has a mortality rate of 100 %.
Vector Control
The diversity of the epidemiology of the different forms of the disease makes it impossible to control leishmaniasis with a single approach or tool. Infection can be prevented by use of repellents or insecticides against sandflies. The elimination of dogs and other reservoir hosts greatly reduces the risk of infection. Vector control using suitable insecticides are effective against peridomestic transmission.
cutaneous leishmaniasis

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